The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration

Document Type : Original research paper

Authors
Maziar Habibi 1
Saeid Malekzadeh-Shafaroudi 1
Hassan Marashi 1
Nasrin Moshtaghi 1
Mohammadreza Nasiri 1
Saeed Zibaee 2
1 Department of Biotechnology and plant breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Razi Vaccine and Serum Research Institute, Mashhad, Iran.
10.22058/jpmb.2014.11950
Abstract
Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes a serious loss in the production of milk and meat. Therefore, producing an effective recombinant subunit vaccine virus this disease is of great importance. Transient gene expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An Agrobacterium-mediated transient gene expression assay was carried out in spinach (Spinacia oleracea) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via agroinfiltration procedure. The presence of foreign gene and its expression in transformed plants were confirmed through polymerase chain reaction (PCR), real time PCR, protein Dot blot and ELISA. The results obtained in this examination showed quite a high level of gene expression in spinach leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. The procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study.
Keywords
agroinfiltration
FMDV
recombinant vaccine
spinach
VP1
Journal of Plant Molecular Breeding
Volume 2, Issue 2 - Serial Number 2
December 2014
Pages 18-27

  • Receive Date 25 April 2014
  • Accept Date 25 July 2014
  • First Publish Date 01 December 2014