Glomus mosseae symbiosis affects expression of stress-responsive genes in rice under water deficit conditions

Document Type : Original research paper

Authors
Arezoo Pourfarid 1
Ali Pakdin-Parizi 2
Reza Ghorbani-Nasrabad 3
Heshmatollah Rahimian 4
1 Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University
2 Genetics and Agricultural biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
3 Department of Soil Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Department of plant protection, Sari University of agricultural sciences and natural resources, Sari, Iran
10.22058/jpmb.2022.546717.1249
Abstract
Arbuscular mycorrhizal fungi (AMF) symbiosis could mitigate the adverse effects of abiotic stresses in various plants. The aim of this study was to investigate the effect of AMF-inoculation on expression of several stress-responsive genes in two rice cultivars under different water conditions. The seedlings of Tarom-Hashemi and Nemat rice cultivars were transplanted in soil with or without G. mosseae spores. At the tilling stage, the AMF-inoculated (+AMF) and AMF-uninoculated (−AMF) plants were subjected to flooded and water deficit conditions (70% field capacity (FC) and 50%FC). The genes expression was evaluated by qRT-PCR and reported relative to control (flooded, -AMF) plants. The results showed lower expression of osDREB2A in +AMF plants in comparison with –AMF plants under water deficient conditions. The expression of OsPIP1;2 was significantly increased in roots of +AMF to –AMF plants. But, the expression of this gene was decreased in shoots of +AMF and –AMF plants in comparison with control plants. The stress-responsive gene transcripts, OsPIP2;3, OsGH3-8, OsLTP, OsAOS2 and OsADC1 in +AMF rice cultivars was higher than -AMF plants at both water deficit conditions. Expression of OsP5CS in +AMF and –AMF plants was increased in comparison with control plants, though, their differences was not significant. In 70%FC, OsEXP15 gene expression of +AMF and –AMF root plants was increased in comparison with control plants. However, under 50%FC the gene expression was decreased and not changed in -AMF and +AMF plants, respectively. It seems AMF induced changes in rice genes expression may enhance tolerance to water deficit conditions.
Keywords
Arbuscular mycorrhizal fungi
aquaporin
expansin
polyamine accumulation
qRT-PCR
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Journal of Plant Molecular Breeding
Volume 8, Issue 2
December 2020
Pages 61-72

  • Receive Date 12 January 2022
  • Revise Date 15 January 2022
  • Accept Date 16 January 2022
  • First Publish Date 16 January 2022