Enhancing haploid wheat induction efficiency through the wheat × maize cross utilizing silver nitrate and calcium phosphate

Modirrousta, Hamed; Khademian, Raheleh; Bozorgipoor, Reza

doi:10.22058/jpmb.2023.2007085.1279

Enhancing haploid wheat induction efficiency through the wheat × maize cross utilizing silver nitrate and calcium phosphate

Document Type : Original research paper

Authors

Hamed Modirrousta ¹

Raheleh Khademian ¹

Reza Bozorgipoor ²

¹ Department of Genetic and Plant Breeding, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran

² Ministry of Jahad -e-agriculture, agricultural research education and extension organization seed and plant improvement research institute

10.22058/jpmb.2023.2007085.1279

Abstract

This study aimed to assess the efficacy of silver nitrate and calcium phosphate in inducing chromosome elimination and promoting haploid wheat generation in the context of wheat-maize crosses. The experiment explored the impact of various treatments, including non-use (control) and the utilization of silver nitrate (3gr/l) combined with calcium phosphate (0.1gr/l). Additionally, different durations of treatments post-pollination, spanning 48 and 72 hours, were considered. The study evaluated key characteristics such a number florets, number as well as the quantity and percentage of successful seed formations. The findings underscored significant distinctions among the treatments, achieving a level of significance of 1%. The use of silver nitrate in conjunctionwith calcium phosphate for wheat tillers demonstrated an increased propensity for haploid embryo formationSpecifically, maintaining wheat tillers in a solution of silver nitrate and calcium phosphate for 72 and 48 hours, in comparison to the control group, resulted in haploid embryo formations with proportions of 12.53%, 5.60%, and 3.82%, respectively. These results indicate that extended exposure to the silver nitrate-calcium phosphate treatmentenhances the formation of haploid embryos.

Keywords

Haploid

Chromosome elimination

Interspecific hybridization

Embryo rescue

Tissue culture

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