Genome-wide analysis of the HSP90 gene family and their roles in soybean growth and development

Mohammadi, Samira; Sohrevardi, Firouzeh; Nematzade, Ghorbanali

doi:10.22058/jpmb.2024.555256.1256

Genome-wide analysis of the HSP90 gene family and their roles in soybean growth and development

Document Type : Original research paper

Authors

Samira Mohammadi

Firouzeh Sohrevardi

Ghorbanali Nematzade

Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)

10.22058/jpmb.2024.555256.1256

Abstract

Heat shock protein of 90 kDa or HSP90 plays an important dynamic role in regulating biotic and abiotic stresses through multiple functional mechanisms. The present study aimed to perform a comprehensive analysis of the HSP90 gene family in soybean. In total, 20 HSP90 genes from soybean were identified and showed unequal distribution on the 13 chromosomes. The evolutionary tree divided these genes into three main groups based on their subcellular localization. In Group I, nearly all of the HSP90 genes are distributed in the nucleus or cytoplasm. In Group II, the HSP90s were mostly classified in the endoplasmic reticulum. HSP90 genes were exclusively found in the mitochondria or chloroplast in Group III. Phylogenetic relationships have shown that genes in similar subgroups have the same exon-intron structure and number of introns. Glyma14g219700, Glyma17g258700, and Glyma07G207600 were identified as hub proteins based on their high degrees of interaction. In addition, Glyma02g302500, Glyma08g332900, Glyma14g219700, Glyma17g258700, and Glyma18g074100 genes displayed high expression levels in all of the tissues at different developmental stages. These findings provide a complete overview of the GmHSP90 gene family classification and evolution, which can help to identify the functional properties of the HSP90 genes in soybean growth and development.

Keywords

Gene expression profiling

gene structure

heat shock protein

phylogenetic analysis

protein-protein interaction network

subcellular localization

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