Microsatellite-based heterotic grouping of temperate maize (Zea mays L.) inbred lines

Document Type : Original research paper

Authors
Mahnaz Oroojloo 1
Behzad Ahmadi 2
Sara Dezhsetan 1
Mohamadreza Shiri 2
Ali Moghaddam 2
1 Department of Genetic and Plant Production, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Maize and Forage Crops Research, Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
10.22058/jpmb.2024.2042682.1308
Abstract
Maize (Zea mays L.) is an essential cereal crop globally, with breeding efforts aiming to develop high-yielding hybrids through heterotic patterns. This study assessed the feasibility of classifying 51 maize inbred lines into the heterotic groups using 30 Simple Sequence Repeat (SSR) markers. Out of the 30 marker pairs tested, 28 displayed polymorphism, producing a total of 68 alleles, ranging from 2 to 4 alleles per locus, with an average of 2.43 alleles per locus. The primers umc2152 and Bnlg1194 exhibited the highest number of alleles, while the marker mmc0481 had the highest allele frequency. Polymorphic Information Content (PIC) values ranged from 0.08 to 0.93, with an average value of 0.56. The highest ∆K value resulted in the classification of the inbred lines into five distinct heterotic groups. The findings suggest that SSR markers effectively reveal significant genetic diversity, making them valuable tools for the classification of maize inbred lines. This categorization can assist in identifying heterotic patterns and predicting heterosis for future hybrid production.
Keywords
Maize
heterotic group
genetic diversity
simple sequence repeats
Subjects
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Journal of Plant Molecular Breeding
Volume 12, Issue 1
June 2024
Pages 60-69

  • Receive Date 09 October 2024
  • Revise Date 26 October 2024
  • Accept Date 27 October 2024
  • First Publish Date 27 October 2024