Identification and expression analysis of Oleosin gene family in walnut (Juglans regia L.)

Hashemipetroudi, Seyyed Hamidreza; Ahmadi, Fatemeh

doi:10.22058/jpmb.2025.2029871.1317

Identification and expression analysis of Oleosin gene family in walnut (Juglans regia L.)

Document Type : Original research paper

Authors

Seyyed Hamidreza Hashemipetroudi ¹

Fatemeh Ahmadi ²

¹ Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari

² Department of Horticultural Science and Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

10.22058/jpmb.2025.2029871.1317

Abstract

Oleosins as structural proteins play a crucial role in stabilization the oil-bodies, their size, number, and content. Here, exon/intron structure, conserved motifs/ domains, and expression patterns of Oleosin gene family were identified in diploid crop Juglans regia L. This study found seven non-repetitive JrOleosin genes through a genome-wide analysis, displaying remarkable structural and physicochemical differences. Our phylogenetic analysis of the 46 oleosin genes revealed that how these genes are related, highlighting that they share conservations while also having unique divergences across different species. The JrOleosin intronless genes (IGs) might be examples of how evolution adapts to enhance the accuracy and effectiveness of gene expression. Transcriptome analysis showed that the JrOleosin genes were spatially and temporarily regulated with the highest transcript found in seeds and fruits. This suggests they are vital for storing lipids during these developmental stages. These results help us to better understand the features of the JrOleosins gene family, especially their structure and expression. Future research should concentrate on revealing how oleosin expression is controlled and how these proteins interact with others linked to lipid synthesis during seed development.

Keywords

Oil-body

oleosin

meta-analysis

walnut, genome-wide

Subjects

Plant bioinformatics

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