Green synthesis of gold nanoparticles using aqueous extract of Haplophyllum canaliculatum Boiss and its antioxidant activity

Ahadi, Niloofar; Larijani, Kambiz; Ghaderi, Lida; Asen, Parvin

doi:10.22058/jpmb.2025.2058107.1336

Green synthesis of gold nanoparticles using aqueous extract of Haplophyllum canaliculatum Boiss and its antioxidant activity

Document Type : Original research paper

Authors

Niloofar Ahadi ¹

Kambiz Larijani ¹

Lida Ghaderi ²

Parvin Asen ³

¹ Department of Chemistry, Science and Research Branch, Islamic Azad University , Tehran , Iran

² Faculty of Medicinal Plants, Amol University of Special Modern Technologies, Amol, Iran

³ Department of Nano Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol , Iran

10.22058/jpmb.2025.2058107.1336

Abstract

Green synthesis of gold nanoparticles (AuNPs) is increasingly popular due to their broad potential applications. Hence, this research reports the synthesis of AuNPs by reducing of HAuCl₄ using an aqueous extract of Haplophyllum canaliculatum Boiss as both a reducing and stabilizing agent. In addition, the antioxidant activity of as-prepared AuNPs was investigated. The physicochemical properties of AuNPs were characterized by UV-visible (UV-Vis) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM). The UV-Vis spectra displayed a surface plasmon resonance band at 523 nm, which confirmed the formation of AuNPs. Also, the FESEM image revealed that the particle sizes of the AuNPs ranged from 15 to 25 nm. In addition, the Debye-Scherrer equation showed a particle size of 16.4 nm from the XRD result. Furthermore, the biosynthesized AuNPs exhibited significant antioxidant activity (P< 0.05) against DPPH free radicals’ scavenger in a concentration-dependent manner . The IC₅₀ value of the AuNPs was obtained as 44.42 µg mL^-1. The antioxidant activity of the AuNPs likely stems from secondary metabolites of the H. canaliculatum extract, such as quinoline alkaloids. These findings offer a promising approach for developing biosynthesized AuNPs for potential pharmaceutical applications.

Keywords

Green synthesis

gold nanoparticles

Haplophyllum canaliculatum

antioxidant activity

Subjects

Plant biochemical and regulatory networks

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